Fig. 6
From: Hsp90β promoted endothelial cell-dependent tumor angiogenesis in hepatocellular carcinoma
Immunohistochemical analysis of VEGFRs expression and tumor vascularization in a HCC xenograft model. a Control group mice subcutaneously received PLC cell suspensions. Hsp90β group mice subcutaneously received a suspension of PLC cells transfected with pcDNA-Hsp90β plasmids. NVP-BEP800 group mice subcutaneously received a suspension of PLC cells and were treated by intragastric administration with NVP-BEP800 (30 mg/kg/day). b Tumor sizes were measured every 2 days and tumor volumes were calculated. c, d To check the effect of Hsp90β and NVP-BEP800 on cell proliferation, immunohistochemical analysis of Ki67 was performed with the tumor issues. The intensity of Ki67-positive cell was calculated with Image J software. e, f VEGFR1 and VEGFR2 expression in tumor tissue of the control group, Hsp90β group, and NVP-BEP800 group mice. g, h Analysis of MVD based on CD31 staining of tumor tissue by Image J software showed that vessel number significantly increased in the Hsp90β group and that vessel number decreased in the NVP-BEP800 group. *P < 0.05, **P < 0.01