Fig. 3

Silence of IGF2BP3 exerts anti-oncogenic role in GC. a Enrichment plots of gene expression signatures for common cancer genes (LIU_COMMON_CANCER_GENES) (P < 0.001), cell proliferation (CHIANG_LIVER_CANCER_SUBCLASS_PROLIFERATION_UP) (P < 0.001) and cell cycle progression (CELL_CYCLE_GO_0007049) (P = 0.004) according to IGF2BP3 mRNA expression in a published cohort (NCBI/GEO/GSE62254, n = 300). The barcode plot indexed the position of the genes in each gene set. Red and blue colors indicated high and low level of IGF2BP3. ES, enrichment score; NES, normalized enrichment score. b mRNA expression of IGF2BP3 after siRNA-mediated knockdown in MKN28 and SGC-7901 cells (**, P < 0.001). c Knocking down IGF2BP3 reduced cell growth in a 4-day MTT proliferation assay in MKN28 and SGC-7901 cells (**, P < 0.001). d Monolayer colony formation of GC cells was suppressed by siIGF2BP3 (**, P < 0.001). e IGF2BP3 knockdown inhibited cell invasive ability (**, P < 0.001). f Expression of related cell cycle regulators and apoptotic markers by Western blot analysis. CDK4, CDK6 and p-Rb were down-regulated, while p21 and p27 showed uniformly elevated expression in IGF2BP3-depleted cells. g Flow cytometry analysis of IGF2BP3 knockdown transfectants together with scramble siRNA transfectants as control. Two independent experiments were performed and the representative one was shown in the bar chart