Fig. 3

mTORC1 phosphorylate Skp2 on Ser64. a 293 T cells were contransfected with Flag-Skp2-S64A and Myc-mTOR-WT for 48 h. The cell lysates were analyzed by Western blot. b, c 293 T cells were transfected with the indicated plasmids and harvested for co-immunoprecipitation experiments. d The 293 T cell were treated IGF-1 at various time points after starvation 12 h. The cell lysates were analyzed by Western blot. e The 293 T cell were treated with rapamycin at various time points. The cell lysates were analyzed by Western blot