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Fig. 2 | Molecular Cancer

Fig. 2

From: Cripto-1 acts as a functional marker of cancer stem-like cells and predicts prognosis of the patients in esophageal squamous cell carcinoma

Fig. 2

CR-1 knockdown significantly represses the self-renewal and tumorigenicity of EC109 cells. a CR-1 knockdown (shCR1) dramatically decreased the expression of stemness-related transcription factors Sox2, Oct4 and Nanog. b Limiting dilution assay showed that CR-1 knockdown markedly suppressed sphere formation. c. CR-1 knockdown significantly repressed colony formation. d Representative images showed that CR-1 knockdown decreased tumor formation in a xenografted tumor mouse model. e Quantitative analysis of the weight of xenografts formed by EC109 cells (2 × 104 cells and 2 × 105 cells, respectively). f H&E staining confirmed the origination of xenografts and IHC staining confirmed that xenografts derived from shCR-1 cells express less CR-1 compared to Mock cells. * indicates p < 0.05; ** indicates p < 0.01

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Molecular Cancer

ISSN: 1476-4598