Fig. 2
From: Human germ/stem cell-specific gene TEX19 influences cancer cell proliferation and cancer prognosis
Analysis of TEX19 depleted SW480 cells: a TEX19 depleted SW480 cells do not have cleaved caspase-3 indicting there is no measurable apoptosis. b TEX19 depleted SW480 cells (lower image) do not form full spheres and exhibit no staining with β-galactosidase indicating that they are not undergoing lysosome-dependent senescence (upper image shows SW480 spheres in the absence of TEX19 siRNA staining positive for β-galactosidase due to natural senescence of internal cells; bar = 250 μm). c FACS of TEX19 siRNA treated SW480 cell populations (a minimum of 10,000 cells were analyzed in each case). d TEX19 siRNA treated cells have a lower proportion of the population in G2 and a higher proportion in S phase. e Western blot of whole cell extracts of SW480 cells depleted for TEX19 have lower levels of phosphor-histone H3 indicating fewer cells in mitosis. f Western blot and RT-qPCR analysis demonstrating TEX19 depletion (for data shown in a and c-e)