Fig. 1

The expression of A2AR is increased and correlated with clinicopathological parameters in human HNSCC. a Confocal Immunofluorescence images of A2AR in HNSCC. A2AR expression is located on the tumor infiltrating immune cells (scale bar = 10 μm). b Representative HE and immunohistochemical staining of A2AR in human normal oral mucosa and HNSCC tissue (scale bar = 100 μm). c Quantification of histoscore of A2AR expression in normal oral mucosa (Mucosa), oral epithelial dysplasia (Dys), and primary HNSCC (PH) tissue. The expression of A2AR was significantly elevated in primary HNSCC tissues as compared with normal oral mucosa or oral epithelial dysplasia (Mean ± SEM, ***, P < 0.001, One way ANOVA with post Tukey test). d-f The expression of A2AR was correlated with d advanced pathological grade, e larger tumor size and f positive lymph node status in primary HNSCC (Mean ± SEM, **, P < 0.01, ***, P < 0.001, One way ANOVA with post Tukey test or unpaired t test). g Kaplan-Meier survival analysis indicated that high expression of A2AR represented unfavorable prognosis of HNSCC patients (P = 0.0383). h The expression of A2AR was significantly increased in recurrent HNSCC (recurrence, Mean ± SEM, *, P < 0.05, unpaired t test). i The expression of A2AR was significantly increased in HNSCC with induction chemotherapy (post TPF, Mean ± SEM, **, P < 0.01 unpaired t test). All precise P value and the Mean ± SEM was displayed in Table 1