Fig. 4

PDKs are crucial for PDH phosphorylation. A375 cells were transfected with siRNA against all four PDK isoforms (PDK1–4; 25 nM siRNA for each isoform) or 100 nM of scrambled control; after 24 h cells were incubated with PLX4032 (1 μM) for another 24 h. a qPCR analysis of PDK1–4 mRNA levels to show the efficiency of the siRNA knock-down of each siRNA and (b) corresponding western blot analysis. c Western blot analysis of A375 treated with PLX4032 (1 μM for 24 h), DCA (20 mM for 48 h) or both drugs. Error bars represent the standard deviation of three biological replicates. For each western blot experiment, one representative of three biological replicates is shown. Statistical significance was determined using paired Student’s t-tests. *p > 0.05, **p > 0.01, ***p > 0.001. 1: Untransfected; 2: scrambled; 3: PDKs siRNA