Fig. 2

Oct4 promoted oncogenic lncRNAs transcription through activating promoter of NEAT1 and enhancer of MALAT1 and UCA1. a Schematic diagram depicting the construction of promoter activity assay of NEAT1 (upper left). The wild-type (WT) and mutation (Mut) sites at the Oct4 consensus region are shown (lower left). Dual luciferase assay performed in A549 (middle) and CL1–0 (right) cells. b, c Schematic diagram depicting the construction of enhancer activity assay (upper left). The minimal promoters reported were inserted upstream of the luciferase reporter, while the Oct4 binding ChIP-seq regions were inserted downstream as the enhancer plasmids. The WT and Mut sites at the Oct4 consensus region are shown (lower left). Cells were transfected with vector or Oct4 plasmids and luciferase plasmids of NEAT1 (a) MALAT1 (b) or UCA1 (c). Data are mean ± SEM. P-values were determined by two-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001