Fig. 3

Reciprocal restrain between miR-103a-3p and linc00152, down-regulated of linc00152 hindered the malignant behavior of GSCs by target miR-103a-3p. a qRT-PCR analysis revealed the negative correlation between miR-103a-3p and linc00152 expression in glioma cells. **P < 0.01 vs. pre-NC group; ^##P < 0.01 vs. anti-NC group; **P < 0.01 vs. sh-NC group. b Linear regression analysis was done to each individual linc00152 and miR-103a-3p expression, the slope was −4.243, **P < 0.01. c Schematic representation of the putative binding site for linc00152 and miR-103a-3p, and the contrivable mutant sequence indicated for the reporter assay. d The relative luciferase activities were restrained in the HEK-293 T cells co-transfected with vector linc00152-wt and miR-103a-3p. *P < 0.05. vs. linc00152-wt + miR-103a-3p-NC group; ^#P < 0.05. vs. linc00152-mut + miR-103a-3p-NC group. e The CCK-8 assay was conducted to evaluate the effects of linc00152 and miR-103a-3p on the proliferation of GSCs. f-g The capacities of migration and invasion were detected through transwell assays on GSCs. h Flow cytometry analysis of GSCs in groups according to linc00152 and miR-103a-3p expression. *P < 0.05 vs. sh-NC + pre-NC group; ^#P < 0.05 vs. sh-linc00152 + pre-NC groups;^&P < 0.05 vs. sh-NC + pre-miR-103a-3p group; ^▲P < 0.05 vs. sh-NC + anti-NC group; ^★P < 0.05 vs. sh-linc00152 + anti-NC group;^▼P < 0.05 vs. sh-NC + anti-miR-103a -3p group. Scale bars, 20 μm. For a, c, d, e and g, data are presented as the mean ± SD (n = 5, each group)