Fig. 3

MRCCAT1 knockdown suppresses ccRCC cells proliferation, migration and invasion in vitro. a Relative expression of MRCCAT1 in 786-O and Caki-1 cells after transfecting with MRCCAT1 shRNAs compared with negative control (NC). b CCK-8 assay of MRCCAT1 knockdown (shRNAs) and NC ccRCC cells at indicated times. c, d Upper: representative images of the wound-healing assays of MRCCAT1 knockdown with shRNAs or NC in 786-O (c) and Caki-1 cells (d) photographed at indicated times after scratching. Scale bar = 800 μm. Lower: relative breath after migration was measured and calculated among the groups. e, f Left: Transwell assays were performed to evaluate cell invasion following MRCCAT1 knockdown or NC in 786-O cells (e) and Caki-1 cells (f). Scale bar = 200 μm. Right: the statistical graph indicates the number of cells averaged from 8 random high power fields. The results are presented as the mean ± SD from three independent experiments; *P < 0.05, **P < 0.01 by Student’s t-test