PRC1 knockdown induces G2/M phase arrest and apoptosis in vitro.
a Cell-cycle analysis was determined in A549, SPC-A1, and H1299 cells transduced with shPRC1 or shControl. The DNA content was quantified by flow cytometric analysis. The percentage of cells in each population was shown as the mean ± S.D. from three independent experiments. Student’s t-test **P < 0.01, ***P < 0.005. b Western blot analysis of G2/M transition-related proteins in A549, SPC-A1, and H1299 cells transduced with shPRC1. Similar experiments were repeated at least twice with similar results. c Apoptosis in A549 and SPC-A1 cells transduced with shPRC1 was performed by flow cytometry and the apoptotic index was defined as the percentage of apoptotic cells. **P < 0.01. d Caspase-3, PARP, Bcl-xl, Bcl2, and Bax were evaluated by western blotting in both cell lines after treatment with shPRC1. Similar experiments were repeated at least twice with similar results