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Fig. 2 | Molecular Cancer

Fig. 2

From: Long non-coding RNA linc00673 regulated non-small cell lung cancer proliferation, migration, invasion and epithelial mesenchymal transition by sponging miR-150-5p

Fig. 2

Effects of linc00673 on lung cancer cell apoptosis, viability, migration and invasion. a. Validation of siRNA knockdown efficiency in A549 cells as determined by qRT-PCR. b. Validation of siRNA knockdown efficiency in H1975 cells as determined by qRT-PCR. c. Validation of pcDNA3.1-linc00673 over-expression vector in H1703 cells as determined by qRT-PCR. d. Flow cytometric analysis of apoptosis in si-NC or si-L3 transfected A549 and H1975 cells. e. Expression of PARP and pro-Caspase 3 in si-NC or si-L3 transfected A549 cells as determined by western blot. f. CCK-8 proliferation assay in si-NC or si-L3/si-L5 transfected A549 and H1975 cells. g. EdU proliferation assay in si-NC or si-L3/si-L5 transfected A549 and H1975 cells. h. Wound scratch assay in si-NC or si-L3/si-L5 transfected A549 and H1975 cells and in pcDNA3.1-linc00673 transfected H1703 cells. i. Transwell invasion assay in si-NC or si-L3/si-L5 transfected A549 and H1975 cells and in pcDNA3.1-linc00673 transfected H1703 cells. j. Tumor growth in NOD/SCID mice with tail vein injection of linc00673-shRNA or sh-nc transfected A549 cells. Error bars indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.005

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Molecular Cancer

ISSN: 1476-4598