Fig. 3

miR-210-3p promotes EMT, invasion and migration in PCa cells in vitro. a Gene set enrichment analysis (GSEA) revealed that miR-210-3p expression significantly and positively correlated with the EMT signatures. b Real-time PCR analysis of miR-210-3p expression inVCaP and C4-2B cells transduced with miR-210-3p or transfected with anti-miR-210-3p compared to controls. Transcript levels were normalized by U6 expression. Error bars represent the mean ± s.d. of three independent experiments. *P < 0.05. c Silencing miR-210-3p converted a stick-like or long spindleshaped mesenchymal profile to a cobblestone-like or a short spindle-shaped epithelial morphology in VCaP and C4-2B cells treated with TGF-β (5 ng/ml for 72 h). d Overexpression of miR-210-3p decreased E-cadherin expression and increased Vimentin and Fibronectin expression in VCaP and C4-2B cells; while silencing miR-210-3p increased E-cadherin expression and decreased Vimentin and Fibronectin expression in VCaP and C4-2B cells. α-Tubulin served as the loading control. e and f Overexpression of miR-210-3p enhanced, while silencing miR-210-3p suppressed invasion (e) and migration (f} abilities in VCaP and C4-2B cells. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05