Fig. 3

SOCS3 is the direct target of miR-222-3p. a SOCS3 expression was examined by western blotting. b Sequence alignment of miR-222-3p with reverse complementary miR-222-3p (rcmiR-222-3p), SOCS3 (SOCS3 3’UTR), mutant rcmiR-222-3p (mrcmiR-222-3p), and mutant SOCS3 (mSOCS3 3’UTR). c SOCS3 expression in A549-P cells after transfection with miR-222-3p mimic or inhibitor was examined by western blotting. d The promoter activities of SOCS3 were measured using the dual-Luciferase reporter assay after transfection of A549-P cells alone or in the presence of miR-222-3p mimic or inhibitor. Data are representative of three experiments. Error bars represent ±SD. *P < 0.05; **P < 0.01 vs. vector alone group. e Expression levels of SOCS3/JAK2/Stat3 and Bcl-2/Bax after transfection with miR-222-3p mimic and inhibitor were assessed by western blotting