Fig. 1

MEG2 is downregulated in human gastric cancer and functions as a tumour suppressor gene to suppress the proliferation and migration of gastric cancer cells. a and b Western blot analysis of MEG2 protein in 20 paired human gastric cancer specimens (GC) and normal adjacent gastric tissue (GN) samples. a representative image; b quantitative analysis. c Western blot analysis of MEG2 protein in 5 human GC cell lines and 1 human gastric epithelial cell line. d Western blot analysis of MEG2 protein in MGC803 cells transfected with MEG2 siRNA, control siRNA, MEG2 plasmid and control plasmid. e Cell proliferation assays were performed after the transfection of MGC803 cells with MEG2 siRNA and control siRNA in equal doses. f Cell proliferation assays were performed after the transfection with MEG2 plasmid and control plasmid in equal doses. g and h Transwell migration assays were performed after the transfection with MEG2 siRNA, control siRNA, MEG2 plasmid and control plasmid. g representative images; h quantitative analysis. * P < 0.05; ** P < 0.01; *** P < 0.001