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Fig. 3 | Molecular Cancer

Fig. 3

From: MEG2 is regulated by miR-181a-5p and functions as a tumour suppressor gene to suppress the proliferation and migration of gastric cancer cells

Fig. 3

miR-181a-5p directly regulates MEG2 expression. a and b Quantitative RT-PCR analysis of miR-181a-5p levels in MGC803 and SGC7901 cells transfected with pre-miR-181a-5p, pre-miR-control, anti-miR-181a-5p or anti-miR-control in equal doses. c-f Western blot analysis of MEG2 protein in MGC803 and SGC7901 cells transfected with pre-miR-181a-5p, pre-miR-control, anti-miR-181a-5p or anti-miR-control. C and D: representative image; E and F: quantitative analysis. g Quantitative RT-PCR analysis of MEG2 mRNA levels in MGC803 and SGC7901 cells transfected with pre-miR-181a-5p, pre-miR-control, anti-miR-181a-5p or anti-miR-control in equal doses. h Direct binding of the MEG2 3′-UTR by miR-181a-5p. Firefly luciferase reporters containing the wild-type (WT) or mutant (MUT) form in the MEG2 3′-UTR were transfected into MGC803 cells with pre-miR-181a-5p, pre-miR-control, anti-miR-181a-5p or anti-miR-control. Luciferase assays were performed 24 h after transfection. Firefly luciferase values were normalized to β-galactosidase activity, and the results were plotted as relative luciferase activity. The luciferase activity in the control cells was set as 1. *P < 0.05; **P < 0.01; ***P < 0.001

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