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Fig. 5 | Molecular Cancer

Fig. 5

From: MEG2 is regulated by miR-181a-5p and functions as a tumour suppressor gene to suppress the proliferation and migration of gastric cancer cells

Fig. 5

Effects of miR-181a-5p and MEG2 on the growth of gastric cancer xenografted tumours in vivo. a Flowchart of experimental layout and representative images of xenografted tumours from implanted mice. MGC803 cells were divided into four groups and were infected with control lentivirus, miR-181a-5p overexpression lentivirus, MEG2 overexpression plasmid, or miR-181a-5p overexpression lentivirus plus MEG2 overexpression plasmid. These differentially treated MGC803 cells were subcutaneously implanted into 5-week-old SCID mice (1 × 107 cells per mouse and 5 mice per group). After 21 days, tumour growth was evaluated. b Quantitative analysis of xenografted tumour weights. c Quantitative RT-PCR analysis of miR-181a-5p expression levels in xenografted tumours. d Quantitative RT-PCR analysis of MEG2 mRNA expression levels in xenografted tumours. e Representative image of western blot analysis of MEG2 protein expression levels in xenografted tumours. * P < 0.05; ** P < 0.01; *** P < 0.001

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Molecular Cancer

ISSN: 1476-4598