Fig. 6

Oncogenic functions of TINCR. a The expression level of TINCR was examined in breast cancer lines. b Knockdown efficiency of siRNAs targeting TINCR in UACC812 and MDA-MB-453 cells, as determined by qRT-PCR. Quantitative normalization of TINCR was performed in each sample using GAPDH expression as an internal control. The relative levels of TINCR vs. GAPDH were determined by the comparative CT (2^−ΔΔCT) method. c The CCK-8 assay was conducted to measure cell proliferation in UACC812 and MDA-MB-453 cells after the knockdown of TINCR. d TUNEL apoptosis analysis of UACC812 and MDA-MB-453 cells after the knockdown of TINCR. e Flow cytometry apoptosis analysis of UACC812 and MDA-MB-453 cells after the knockdown of TINCR. f Determination of the cell cycle distribution in UACC812 and MDA-MB-453 cells after the knockdown of TINCR via flow cytometry. *** P < 0.0 01;** P < 0. 01; * P < 0.05. Each assay was performed in triplicate