Fig. 5

NMI regulated COX-2 transcription through p300. a TCGA data was analyzed for the correlation of NMI and p300 gene expression levels (n = 576). Red = high, black = medium, green = low. b Negative correlation in the expression of NMI and p300 was determined in TCGA database. c The expression of p300 in A549 cells with NMI knockdown was detected by immunofluorescence. d The subcellular localization of NMI and p300 in H1299 and A549 cells were examined by confocal microscopy. More than 100 cells were inspected per experiment, and cells with typical morphology were presented. e The expression of the nuclear p300 (upper panel), and the level of the nuclear p50 and p65 pulled down by the anti-pan-acetyl antibody (i.e. acetylated p50 and p65 in the nucleus, lower panel) were detected by western blot 48 h after transfection