Skip to main content
Fig. 2 | Molecular Cancer

Fig. 2

From: Long noncoding RNA ZEB1-AS1 epigenetically regulates the expressions of ZEB1 and downstream molecules in prostate cancer

Fig. 2

ZEB1-AS1 promoted the expression of ZEB1. a, effects of ZEB1-AS1 knockdown on ZEB1. ZEB1-AS1 knockdown in DU145 and PC3 prostate cancer cells (ZEBAS1-KD1 and ZEBAS1-KD2, RNAi-CON1 and RNAi-CON2 as negative control) significantly decreased ZEB1-AS1 mRNA level as well as ZEB1 mRNA and protein levels. ZEB1 knockdown (ZEB1-KD1, ZEB1-KD2) significantly decreased the level of ZEB1 but not ZEB1-AS1. b, effects of ZEB1-AS1 re-expression on ZEB1. Adenovirus-mediated ZEB1-AS1 re-expression (AD-ZEBAS1, with AD-CON as negative control) in ZEB1-AS1 knockdown cells significantly recovered ZEB1-AS1 mRNA level as well as ZEB1 mRNA and protein levels. The ZEB1 expression plasmid pc-ZEB1 (pcDNA3.1 as negative control) significantly increased the level of ZEB1 but not ZEB1-AS1 in both ZEB1-AS1 knockdown and ZEB1 knockdown cells

Back to article page

Molecular Cancer

ISSN: 1476-4598