Fig. 3

Knockdown of CASC9 inhibited esophageal cancer cells growth in vitro and in vivo. a Expression of CASC9 in normal esophageal epithelial cell line Het-1A, ESCC cell lines (EC109, EC9706, KYSE450, KYSE150) and other common cancer cell lines. GC, gastric cancer; HCC, hepatocellular carcinoma; CC, colonic cancer; PanC, pancreatic cancer; LC, lung cancer; PC, prostate cancer. CASC9 expression was significantly higher in ESCC cell lines compared to Het-1A and other common cancer cell lines. b Knockdown of CASC9 expression in KYSE150 and KYSE450 using small interfering RNA (si-RNA). SI2 was used in the further study for its stable efficiency. c Knockdown of CASC9 inhibited esophageal cancer cells growth in vitro and in vivo. CCK8 assay was performed to determine the cell growth of ESCC cells transfected with NC and SI2. Tumors developed from sh-CASC9 stably transfected KYSE450cells showed smaller volume and weights. d Edu assay was used to determine cell proliferation of KYSE150 and KYSE450 cells after transfected with si-RNA. The bar chart represented the ratio of Edu-positive cells. e Cell cycle was determined by flow cytometry analysis. The bar chart represented the percentage of cells in G0/G1, S, or G2/M phases respectively. Data were mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001