Fig. 7

Silencing PLPP4 inhibits Ca²⁺-permeable cationic channel in lung carcinoma cells. a FACS analysis showed that silencing PLPP4 decreased intracellular Ca²⁺ in the indicated cells. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. b Western blot analysis of cytoplasmic and nuclear expression of the S54 phosphorylated NFAT1 in the indicated cells. α-Tubulin was used as the loading control and the nuclear protein p84 was used as the nuclear protein marker. c Relative NFAT reporter activity in the indicated cells. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. d Hypothetical model illustrating that activation of Ca²⁺-permeable cationic channel by PLPP4 contributes to proliferation and cell cycle progression in lung cancer cells