Fig. 4
From: Overexpression of lncRNA IGFBP4–1 reprograms energy metabolism to promote lung cancer progression
Downregulation of endogenous lnc-IGFBP4–1 inhibits lung cancer cell proliferation in vitro. GLC-82 cells were transfected with lnc-IGFBP4–1-downregulation vector (sh-Lnc-IGFBP4–1) (or empty vector as control), respectively. a CCK8 assay and b clone formation were conducted to determined the cell proliferation. The results from three independent experiments, showed as mean ± s.d. c Cell-cycle analysis was conducted by flow cytometric analysis and showed cell cycle arrest at the G0 – G1 stage. d Apoptosis was detected by flow cytometry and promoted apoptosis. UL, necrotic cells; UR, terminal apoptotic cells; LR, early apoptotic cells. e and f Migration and invasion capacities determined by Transwell assays. *P < 0.05, **P < 0.01