Fig. 4

Linc-RoR promotes ligand-independent activation of ER via MAPK/ERK pathway. a ERK is activated after 6 days estrogen deprivation. b U0126, the MEK inhibitor, abolishes the activation of ERK and ER in response to estrogen deprivation. gRNA control cells were cultured in E2-free medium for 6 days, and then cells were treated with 20 μM U0126 by 8 h before harvesting for Western blot. c Linc-RoR KO suppresses the activation of ERK induced by estrogen deprivation. d Re-expression of linc-RoR restores the phenotype of ERK activation