Fig. 5
From: Linc-RoR promotes MAPK/ERK signaling and confers estrogen-independent growth of breast cancer
Linc-RoR activates ERK by promoting the degradation of DUSP7. a MEK is activated in response to estrogen deprivation. b Detection of MEK activity in gRNA and RoR KO cells in E2-free condition. Note that there was no difference in MEK activity between gRNA and RoR KO. c Suppression of MKP activity by NSC97397 restores ERK activation in linc-RoR KO cells under E2-free condition. d Linc-RoR KO prevents DUSP7 from dagradation under E2-free conditon. e Linc-RoR KO increases protein stability of DUSP7. MCF-7 gRNA and linc-RoR KO cells were treated with CHX (cycloheximide) at 20 μg/ml and then were harvested for western blot at indicated time points. Half-life curve is on the right