Fig. 4

LncRNA RP11-436H11.5 functions by altering miR-335-5p-BCL-W signals to promote RCC cell proliferation and invasion. a. Protein level of BCL-W (ratio to GAPDH) was determined by immunoblot analysis after cotransfection with lncRNA RP11-436H11.5-shRNA and miR-335-5p inhibitor. b. Adding oemiR-335-5p partially rescued the growth of A498 cells transfected with oelncRNA RP11-436H11.5 by a cell proliferation assay. c. Adding miR-335-5p inhibitor partially reversed the growth of 786-O cells transfected with shlncRNA RP11-436H11.5 by a cell proliferation assay. d. A chamber-transwell invasion assay was performed using A498 cells with four groups (control, oelncRNA RP11-436H11.5, oemiR-335-5p, and oelncRNA RP11-436H11.5 + oemiR-335-5p). The invaded cells were counted in 10 randomly chosen microscopic fields (100×) of each experiment and pooled. e. A chamber-transwell invasion assay was performed using OSRC-2 cells with four groups (control, shlncRNA RP11-436H11.5, miR-335-5p inhibitor, and shlncRNA RP11-436H11.5 + miR-335-5p inhibitor). The invaded cells were counted in 10 randomly chosen microscopic fields (100×) of each experiment and pooled. b, c, d and e. Each sample was run in triplicate and in multiple experiments for mean ± SEM. *P < 0.05, **P < 0.01 compared to controls