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Fig. 3 | Molecular Cancer

Fig. 3

From: Hdac7 promotes lung tumorigenesis by inhibiting Stat3 activation

Fig. 3

HDAC7 knockdown suppresses growth of human lung cancer cells. a, Representative Western blot of HDAC7 protein levels of human lung cancer cells, H1299 and A549, expressing shHDAC7 and scramble shRNA. b. The growth curves of human lung cancer cells, A549 and H1299, infected with lenti-shHDAC7 and control viruses. Cell growth was evaluated by MTT assay. c-d. HDAC7 knockdown induces apoptosis of human lung cancer cells. Representative flow cytometry of H1299/shHDAC7 and the control cells stained with Annexin V and 7AAD (c) and the statistical analyses (d) of FACS in c. Annexin V positive cells were counted as apoptotic cells. e-f. HDAC7 knockdown inhibits tumorigenecity of human lung cancer cells. Representative photographs of the soft agar colony formation assay for lung cancer cell lines, H1299 and A549, expressing shHDAC7 and scramble shRNA (e), and the statistical analyses (f) of the results of colony numbers formed in soft agar in e. Values in (b, d and f) represent the means ± SD. of three separate experiments. **, p < 0.01;***, p < 0.001

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