Fig. 5

The SOX3 expression regulated by SOX2OT, miR-194-5p and miR-122. a Real-time PCR and (b) Weatern blot assay were used to detect the SOX3 expression after SOX2OT knockdown. c Real-time PCR and (d) Weatern blot assay were used to detect the SOX3 expression after miR-194-5p over-expression or knockdown. e Real-time PCR and (f) Weatern blot assay were used to detect the SOX3 expression regulated by SOX2OT and miR-194-5p. g The predicted miR-194-5p binding sites in the 3’UTR region of SOX3 (SOX3–3’UTR-Wt) and the designed mutant sequence (SOX3–3’UTR-Mut) are indicated. Relative luciferase activity was conducted after cells were transfected with SOX3–3’UTR-Wt or SOX3–3’UTR-Mut. Data were presented as the mean ± SD (n = 5,each group).^** P < 0.01 vs. SOX3–3’UTR-Wt + Agomir-194-5p-NC group. h Real-time PCR and (i) Weatern blot assay were used to detect the SOX3 expression after miR-122 over-expression or knockdown. j Real-time PCR and (k)Weatern blot assay were used to detect the SOX3 expression regulated by SOX2OT and miR-122. l The predicted miR-122 binding sites in the 3’UTR region of SOX3 (SOX3–3’UTR-Wt) and the designed mutant sequence (SOX3–3’UTR-Mut) are indicated. Data were presented as the mean ± SD (n = 5,each group). ^** P < 0.01 vs. SOX3–3’UTR-Wt + Agomir-122-NC group