Fig. 2
From: Bi-directional exosome-driven intercommunication between the hepatic niche and cancer cells
Cancer exosomes enhance seeding but suppress outgrowth in an all human 3D liver tissue. (a) Schematic of the liver Microphysiological System (MPS). Magnified view of the wells with (upper right) and without (lower right) priming with MDA-231 derived exosomes. (b) Timeline of the experiment to determine seeding efficiency of MDA-231 cells (500 cells) into the liver MPS (total 1.2 million cells). (c) Quantification of the RFP+ MDA-231 cells in the MPS 24 h after seeding, with (d) representative (right) photomicrographs. (e) Timeline of the experiment to determine post-seeding outgrowth in the liver MPS. (f) Quantification of the cells 12 days after seeding, with (g) representative (right) photomicrographs. C and E are mean ± s.e.m. of three experiments each performed in duplicate. * P < 0.05