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Table 1 EVs biomarkers for pancreatic cancer diagnosis

From: Extracellular vesicles as mediators of the progression and chemoresistance of pancreatic cancer and their potential clinical applications

Biomarker Sample Sensitivity and specificity Methods Reference
miR-17-5p, miR-21 Serum:22 PCs, 6 benign pancreatic tumors, 7 ampullary carcinomas, 6 CPs, 8 healthy donors 72.7% and 92.6% for miR-17-5p 95.5% and 81.5% for miR-21 Ultracentrifugation for exosome isolation; RT-PCR for miRNA screening [98]
miR-550 Media from the PANC1 cell line Not mentioned SAW for exosomes lysis; ion-exchange nanomembrane sensor for miRNA detection [99]
miR-10b Plasma:3 PCs, 3 CPs, 3 healthy donors Not mentioned Ultracentrifugation for exosome isolation; LSPR-Based sensor for miRNA quantification [100]
miR-10b, miR-21, miR-30c, miR-181a, miR-let7a Blood:29 PCs, 11 CPs, 6 normal donors Sensitivity:100% Specificity:100% For all these biomarkers Ultracentrifugation for exosome isolation; RT-qPCR for miRNA detection [102]
miR-1246, miR-4644, miR-3976, miR-4306 and CD44v6,Tspan8,EpCAM, MET, CD104 Serum: 131 PCs, 25 CPs, 22 benign pancreatic tumors, 12non-PCs, 30 healthy donors Sensitivity:100% Specificity:80% With 93% for excluding non-Pa-malignancies Ultracentrifugation for exosome isolation; RT-PCR for miRNA detection; flow-cytometry for protein analysis [103]
miR-1246, miR-4644 saliva:12 pancreatobiliary tract cancer patients, 13 healthy donors 66.7% and 100% for miR-1246 75.0% and 76.9% for miR-4644 Total Exosome Isolation
Reagent for exosoems isolation;RT-qPCR for miRNA detection
[105]
Apbblip, Aspn, BCO31781, Daf2, Foxp1, Gng2,Incenp Salivary glands from PC mouse model Not mentioned Ultracentrifugation for exosome isolation; Western-blotting for protein anaysis [106]
MIF Plasma: 5 mice with PanIN, 8 mice with PC, 6 heallthy mice Not mentioned ultracentrifugation for exosome isolation; ELISA for MIF measurement [3]
Glypican-1 Serum: 32 breast cancer, 190 PCs, 100 healthy donor Sensitivity:100% Specificity:100% Ultracentrifugation for exosome isolation;ultraperformance liquid chromatography-mass spectrometry(UPLC-MS) for protein evaluation [101]