Table 1 EVs biomarkers for pancreatic cancer diagnosis

miR-17-5p, miR-21

Serum:22 PCs, 6 benign pancreatic tumors, 7 ampullary carcinomas, 6 CPs, 8 healthy donors

72.7% and 92.6% for miR-17-5p 95.5% and 81.5% for miR-21

Ultracentrifugation for exosome isolation; RT-PCR for miRNA screening

[98]

miR-550

Media from the PANC1 cell line

Not mentioned

SAW for exosomes lysis; ion-exchange nanomembrane sensor for miRNA detection

[99]

miR-10b

Plasma:3 PCs, 3 CPs, 3 healthy donors

Not mentioned

Ultracentrifugation for exosome isolation; LSPR-Based sensor for miRNA quantification

[100]

miR-10b, miR-21, miR-30c, miR-181a, miR-let7a

Blood:29 PCs, 11 CPs, 6 normal donors

Sensitivity:100% Specificity:100% For all these biomarkers

Ultracentrifugation for exosome isolation; RT-qPCR for miRNA detection

[102]

miR-1246, miR-4644, miR-3976, miR-4306 and CD44v6,Tspan8,EpCAM, MET, CD104

Serum: 131 PCs, 25 CPs, 22 benign pancreatic tumors, 12non-PCs, 30 healthy donors

Sensitivity:100% Specificity:80% With 93% for excluding non-Pa-malignancies

Ultracentrifugation for exosome isolation; RT-PCR for miRNA detection; flow-cytometry for protein analysis

[103]

miR-1246, miR-4644

saliva:12 pancreatobiliary tract cancer patients, 13 healthy donors

66.7% and 100% for miR-1246 75.0% and 76.9% for miR-4644

Total Exosome Isolation

Reagent for exosoems isolation;RT-qPCR for miRNA detection

[105]

Apbblip, Aspn, BCO31781, Daf2, Foxp1, Gng2,Incenp

Salivary glands from PC mouse model

Not mentioned

Ultracentrifugation for exosome isolation; Western-blotting for protein anaysis

[106]

MIF

Plasma: 5 mice with PanIN, 8 mice with PC, 6 heallthy mice

Not mentioned

ultracentrifugation for exosome isolation; ELISA for MIF measurement

[3]

Glypican-1

Serum: 32 breast cancer, 190 PCs, 100 healthy donor

Sensitivity:100% Specificity:100%

Ultracentrifugation for exosome isolation;ultraperformance liquid chromatography-mass spectrometry(UPLC-MS) for protein evaluation

[101]