Fig. 6

The depletion of HOTAIR suppressed CAF-induced tumor growth and metastasis in vivo. a Representative pseudocolor bioluminescence images of tumor size were obtained on days 7, 14, 21, 28, 35, and 42. Mice bearing orthotopic tumors of MDA-MB-231-Luc cells were transfected with control shRNA or shHOTAIR. Each group included eight mice (n = 8). Primary tumor volumes (b) and representative bioluminescence images (BLI) (c) were evaluated. d Lung metastasis was detected by bioluminescent imaging on days 35 and 42. e Representative photomicrographs of H&E-stained lung tissues. f The expression of HOTAIR was assessed by FISH. g Representative images of the immunohistochemical staining of CDK5, EZH2, ß-catenin, H3K27me3, CDK5RAP1 and EGR-1 in tissues. Scale bars in all images: 15 μm