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Fig. 3 | Molecular Cancer

Fig. 3

From: The microRNA-15a-PAI-2 axis in cholangiocarcinoma-associated fibroblasts promotes migration of cancer cells

Fig. 3

Identification of miR-15a target genes. a Four criteria for finding the candidate target genes of miR-15a. b The expression levels of eight predicted target genes of miR-15a in 6 CCFs compared to 2 SFs. Bars represent mean ± SD of three measurements. c Expression of PAI-2 in miR-15a mimic-transfected C096 cells by real-time PCR. Scrambled miRNAs were used as negative control miRNA. d Expression of PAI-2 in miR-15a inhibitor transfected SFs. *P < 0.05 compared to control. e Expression of PAI-2 in 6 CCFs, 2 SFs and 2 CCA cell lines. Bars represent means ± SD of three measurements. f PAI-2 expression was examined by Western blot analysis in 2 SFs, 5 CCFs, and 2 CCAs. β-actin was used as a loading control

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