Fig. 3

Nischarin Alters Integrin Expression. a WT (n = 20) and Null MEFs (n = 20) were seeded onto Fibronectin coated coverslips (10 μg/ml) overnight then stained with Zyxin and DAPI. # FA per cell and % area covered by FA was acquired using CellProfiler. b Western Blot detection of VASP in 231 and 231 Nisch cells. c Western Blot of VASP and Vinculn in Cos7 and 231 cells with or without Nischarin. d MDA-MB-231 (n = 3) and MDA-MB-231 Nis (n = 3) cells were seeded onto Fibronectin coated coverslip (10 μg/ml) overnight then stained with VASP. # FA per cell and % area covered by FA was acquired using CellProfiler. e WT and Null MEFs were seeded onto Fibronectin coated coverslips (10 μg/ml) overnight then stained with Vinculin (green) and α5 Integrin (red). f Western Blot of Nischarin, ITGA5, pFAK, t-FAK, and Vinculin in WT, HET and Null MEFs. g 231 (n = 50) and 231 Nis (n = 32) cells were seeded on a Fibronectin coated coverslip (10 μg/ml) overnight then stained with SNAKA51 and DAPI. Cell fluorescence was quantitated through Image J. Corrected Total Cell Fluorescence (CTCF) = Integrated Density – (area of selected cell x mean fluorescence). h RT PCR samples of ITGA1, 2, 4 and 7 RNA in 231 and 231 Nisch cells run on a 3% agarose gel. All scale bars indicate 10 μm