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Fig. 3 | Molecular Cancer

Fig. 3

From: Novel evidence for a PIWI-interacting RNA (piRNA) as an oncogenic mediator of disease progression, and a potential prognostic biomarker in colorectal cancer

Fig. 3

Identification of piR-1245 downstream target genes. a HCT116 cells were treated with or without piR-1245 antisense and subsequently performed gene expression microarray analysis. A total of 244 mRNAs were detected to be differentially expressed with a fold change of ≥ 1.5 and a corresponding P < 0.01. Notably, 168 genes were found to be up-regulated, while 76 genes were down-regulated in piR-1245-inhibited cells compared to control cells. b IPA analysis was performed for the upregulated genes to interrogate the function of piR-1245 in CRC. The results confirmed the putative model that activated p53 pathway, which was induced by piR-1245 inhibition, led to cell apoptosis, necrosis, cell death, contact growth inhibition, senescence of cells, and inhibited cell proliferation, colony formation. Furthermore, IPA showed the piR-1245 acts as important regulator of cell death and survival (c) miRANDA and RNA22 tool was used to predict the binding of piR-1245 to potential targets

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Molecular Cancer

ISSN: 1476-4598