Fig. 1

CD47 is a direct target of miR-708. a Set diagram of top 150 context binding scores miRNA, 70 dysregulated miRNAs and the five chosen miRNAs. b Schematic representation of CD47 3’UTR showing the relative positions of six putative miRNA target sites. c Luciferase activity of the wild type or mutant CD47 3’UTR reporter gene in the HEK 293 T cells transfected with the miRNA or control. Each data point represents the mean ± SD from at least three independent experiments (p < 0.05). d-e CCRF-CEM cells were electroporated with miR-708 inhibitor or miRNA inhibitor-NC and miR-708 mimics or mimics-NC, respectively; The levels of miR-708 was assessed by qRT − PCR.U6 was U6 was carried out as endogenous control in each sample. Cell lysates were prepared for western blotting with the antibody against CD47, and the expression of GAPDH served as a loading control. f qRT-PCR analysis revealed the inverse correlation between miR-708 and CD47 expression in T-ALL. High levels of miR-78 were associated with low CD47 expression (P < 0.005). miR-708 and CD47 expression were normalized to U6 small nuclear RNA and GAPDH, respectively