Fig. 2
From: MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47
Enforced expression of miR-708 enabled cell phagocytosis in vitro and inhibited tumor engraftment in vivo. a Real-time PCR (p < 0.001) and (b) western blot analyses of miR-708 expression level and CD47 protein level, respectively, in CCRF-CEM-LV-NC and CCRF-CEM-LV-miR-708 cells. c. CCRF-CEM-LV-NC and CCRF-CEM-LV-miR-708 cells were fluorescently labeled green by CFSE and incubated with THP1-derived macrophages for 3 h and then examined by fluorescence microscopy. Arrows indicate THP1-derived macrophages containing phagocytosed CCRF-CEM cells. d The phagocytic index (number of target cells ingested per 100 macrophages) was determined for the indicated cell lines. Compared with the phagocytic index of CCRF-CEM-LV-NC, the CCRF-CEM-LV-miR-708 shows a remarkably higher phagocytic index. e. CCRF-CEM-LV-NC and CCRF-CEM-LV-miR-708 cells were labeled in the presence of anti-CD47 antibody, incubated with THP1-derived macrophages for 3 h and then examined by fluorescence microscopy. f The phagocytic index (number of target cells ingested per100 macrophages) was determined for the indicated cell lines. The phagocytic index of CCRF-CEM-LV-miR-708 was significantly higher than that of CCRF-CEM-LV-NC (p < 0.05). g-h, Following the subcutaneous inoculation of CCRF-CEM-LV-NC (left) and CCRF-CEM-LV-miR-708 (right) cells into the flanks of NOD-SCID mice, overexpressed miR-708 inhibited the malignant proliferation of CCRF-CEM cells and reduced subsequent tumor size and growth (i) in vivo. Error bars reflect ±SEM (five mice, *, p < 0.05; **, p < 0.01)