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Fig. 4 | Molecular Cancer

Fig. 4

From: miR-190 suppresses breast cancer metastasis by regulation of TGF-β-induced epithelial–mesenchymal transition

Fig. 4

miR-190 regulates TGF-β signaling by targeting SMAD2. a Luciferase reporter analysis of TGF-β signaling activity in miR-190-overexpressing MDA-MB-231 or control cells with or without TGF-β1 treatment. b The expression of pSMAD2 in cells as in (a). c Localization of SMAD2 in miR-190-overexpressing MDA-MB-231 or control cells with TGF-β1 treatment, as determined by immunofluorescence staining. d The predicted binding of miR-190 with SMAD2 3′-UTR. e Dual luciferase reporter system analysis was performed to validate the miR-190 target, SMAD2. f The miR-190 expression in indicated cells as determined by RT-qPCR. g The expression of SMAD2 in indicated cells as determined by western blotting. ***P < 0.001, *P < 0.05

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Molecular Cancer

ISSN: 1476-4598