Fig. 1

MACC1-AS1 is upregulated in gastric cancer (GC) tissues and predicts poor prognosis. a–b RNA-seq data from the TCGA database showed that levels of MACC1-AS1 and MACC1 are elevated in GC tissues, compared to that in normal counterparts; *P < 0.05, ***P < 0.001. c Correlation analysis showing that MACC1-AS1 is positively correlated with MACC1 expression. d qPCR results showing baseline mRNA levels of MACC1-AS1 in GES1 and five GC cell lines; *P < 0.05, **P < 0.01, ***P < 0.001. e In situ hybridization of MACC1-AS1 showed that MACC1-AS is upregulated in GC compared to expression in adjacent normal gastric mucosa tissues (ANTs); ***P < 0.001. f Representative images of MACC1-AS1 expression in GC tissues of different TNM stages. g MACC1-AS1 expression was positively correlated with MACC1 expression in paired non-cancerous and cancer tissues. The horizontal axis of the heatmap shows MACC1-AS1 scores, whereas the vertical axis shows MACC1 scores. The crossing grid indicates the number of cases (based on different colors). h Representative images of successive IHC/ISH staining of MACC1 and MACC1-AS1 expression in GC tumor tissues of different TNM stage. i Frequency of cases of negative, low, and high MACC1-AS1 expression in GC samples categorized by TNM stage. j–o Elevated expression of MACC1-AS1 and MACC1 was found to be associated with reduced disease-free survival (J-L) in stage I–III GC patients and overall survival (m-o) in stage IV patients