Fig. 1
a DANCR expression in 95 cases of osteosarcoma tissues and paired para-tumor tissues were determined by using of qRT-PCR, data were shown as log2 (2-△△Ct). b-d DANCR expression was significantly elevated in patients with advanced stage (b) (***, P < 0.001,****, p < 0.0001), lymph node metastasis (c) (**, P < 0.01,***, P < 0.001,) and distant metastasis (d) (**, P < 0.01,***, P < 0.001, ###, P < 0.001, respectively) with data be shown as △Ct. e DANCR expression was gradually elevated with advanced staging as detected by In situ hybridization analysis, **, P < 0.01, ***, P < 0.001, as comparing with para-tumor tissue group, respectively. f Elevated DANCR was presented in osteosarcoma cell lines contrasted to hFOB 1.19 group, data was normalized to hFOB 1.19 group, ***, P < 0.001. g Kaplan-Meier analyses indicated that overall survival (OS) in the patients with high DANCR was significantly shorter than that in the patients with low DANCR, P = 0.02. Data were shown as mean ± SD from three independent experiments