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Fig. 3 | Molecular Cancer

Fig. 3

From: Long noncoding RNA DANCR, working as a competitive endogenous RNA, promotes ROCK1-mediated proliferation and metastasis via decoying of miR-335-5p and miR-1972 in osteosarcoma

Fig. 3

a The elevated expression of ROCK1 in tissue level were displayed by an IHC detection, ***, P < 0.001, normalized to para-tumor tissue group. b ROCK1 was up-regulated in osteosarcoma tissue and cell lines as determined by a qRT-PCR, **, P < 0.01 and ##, P < 0.01, normalized to para-tumor tissue group and hFOB 1.19 group, respectively. c A positive correlations between DNACR and ROCK1 was confirmed by a Pearson correlation analysis, P < 0.0001. d-e The expression of ROCK1 after DANCR regulation were measured by using of qRT-PCR (d) and western blot (e) in MNNG/HOS and 143B cells, separately. f-g RNAi technique was used to define the function ROCK1 might work on DANCR induced proliferation. Silencing ROCK1 by transfection of ROCK1 shRNA attenuated DANCR-induced facilitation of proliferation in MNNG/HOS (f) and 143B (g) cells, as determined by an EDU assay. h-i A transwell assay was executed to verify the role of ROCK1 in DANCR-induced metastasis in MNNG/HOS (h) and 143B (i) cells. **, P < 0.01, normalized to pcDNA-DANCR + NC shRNA group. Data were shown as mean ± SD from three independent experiments

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Molecular Cancer

ISSN: 1476-4598