Fig. 6

a-b Representative photographs of IVIS images (a), macroscopic appearance (b up) and growth curve (b down) strongly indicated that elevated DANCR (DANCR puro) promoted tumorigenesis opposite to pMSCV puro group. c, e-g Elevation of DANCR (e, confirmed by qRT-PCR) enhanced ROCK1 (c, detected by IHC and western blot, scale bars 50 μm) but restricted miR-335-5p (f, detected by qRT-PCR) and miR-1972 (g, detected by qRT-PCR) expression in the formatted subcutaneous nudes. d h & e staining demonstrated the formation of osteosarcoma nodules, scale bars, 200 μm and 50 μm for magnifications of 100× and 400×, respectively. h-i Elevation of DANCR led to promotion of lung metastasis of osteosarcoma which were presented by the representative photographs of CT scan, macroscopic appearance and HE staining (scale bars 200 μm, magnifications of 100×). j Elevated expression of DANCR in DANCR puro group was confirmed by qRT-PCR detection compared to pMSCV puro group. k-n Elevation of DNACR suppressed miR-335-5p (k, detected by qRT-PCR) and miR-1972 (l, detected by qRT-PCR) but improved ROCK1 (m by IHC, scale bars 50 μm, and N by western blot detection) expression in formatted metastatic nudes. All data were normalized to pMSCV puro group, **P < 0.01 as comparing to pMSCV puro group. Data were shown as mean ± SD from three independent experiments