Fig. 5

HULC promotes autophagy in liver cancer cells. a (left) Chromatin Immunoprecipitation (CHIP) with anti-RNApolII followed by PCR with LC3 promoter primers in Hep3B cell line. IgG CHIP as negative control. LG3 promoter as INPUT. (right) quantitative CHIP analysis. b (left) RT-PCR with LC3 primer in Hep3B cell line. (right) Real-time RT-PCR analysis. c (left) Western blotting with anti-LC3 in Hep3B cell line. β-actin as internal control. (right) density analysis of band. d Western blotting with anti-sirt1 in Hep3B cell line. e Co-Immunoprecipitation (IP) with anti-Ac Ab followed by Western blotting with anti-LC3 in Hep3B cell line. IgG IP as negative control. INPUT refers to Western blotting with. Anti-LC3. f (left) Western blotting with anti-LC3 and anti-Sirt1 in Hep3B cell lines transfected with pcDNA3,pcDNA3-Sirt1,and pcDNA3-Sirt1 mutant respectively. (right) density analysis of band. g (left) Western blotting with anti-LC3 and anti-Sirt1,and RT-PCR with HULC primers in Hep3B cell line. (right) density analysis of band. h Thereal-time PCR detection of mature miR15a in liver cancer cells. i (left) Western blotting with anti-LC3 and RT-PCR with HULC primers in Hep3B cell line. (right) density analysis of band