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Fig. 6 | Molecular Cancer

Fig. 6

From: Long noncoding RNA HULC accelerates liver cancer by inhibiting PTEN via autophagy cooperation to miR15a

Fig. 6

HULC alters gene expression via autophagy. a Co-Immunoprecipitation (IP) with anti-ATG3 followed by Western blotting with anti-LC3 in Hep3B cell line. IgG IP as negative control. INPUT refers to Western blotting with. Anti-LC3. b Western blotting with anti-becline-1 in Hep3B cell line. c Observation for autophagy (LC3-RFP) in liver cancer cells Hep3B cell line. Scale bars, 100 μm. d Western blotting with anti-SAPK/JaK, anti-PKM2, anti-CDK2, anti-Notch1, anti-C-Jun,anti-PTEN, anti-β-catenin in Hep3B cell lines under starvation or transfected with pCMV6-A-GFP, pCMV6-A-GFP-HULC or pCMV6-A-GFP- HULC plus 3-methyladenine (3-MA),respectively

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Molecular Cancer

ISSN: 1476-4598