Fig. 2

Differential expression of CEP55 in normal and cancer derived exosomes. a Immunoblotting for exosomal proteins in normal (NOK368, OK113, NK4) and malignant (SqCC/Y1, CaLH2, Ca1, SVpgC2a, SVFN8) cell-derived exosomes (top panel) and parental cells (bottom panel). ALIX, exosomal protein; Calnexin, endosomal protein; GAPDH and HSC70 were used as loading controls. b Verification of CEP55 antibody specificity using siRNA on SVFN8 cell line which expresses high levels of CEP55. HSC70 was used as a loading control. c RT-qPCR confirmed that siCEP55, but not siCTRL, significantly (***P < 0.001) knocked down the mRNA of CEP55 in SVFN8. d CEP55 protein localisation studying using immunogold-transmission electron microscopy on exosomes derived from normal human plasma, OK113, SVFN8 and SqCC/Y1 as indicated. Open arrow heads indicate CEP55 protein gold labels (< 15 nm diameter including the halo around each black dot). Scale bars represent 30 nm