Fig. 4
From: MASTL induces Colon Cancer progression and Chemoresistance by promoting Wnt/β-catenin signaling
Cell cycle arrest induces apoptosis in MASTL inhibited cells. Control and MASTL knockdown cells were synchronized in serum-free conditions for 72 h, after which they were treated with RO3306 for 16 h. Media was replaced with fresh media for one hour after which cells were collected for (a) Edu cell proliferation assay and quantitation of number of proliferative cells; (b & c) (i) FITC Annexin V staining analysis via FACS. HCT116 and SW620 MASTL knockdown cells showed a significant induction of apoptosis at both early and late phases compared to control cells. (ii) Caspase activity assay further confirmed increased apoptosis in MASTL knockdown cells compared to control cells. For graphs, data represent mean ± SD; * P < 0.01