Fig. 4

SOX2 knockdown inhibits the viability, migration and invasion of glioma cell lines. a The relative expression of SOX2 increases in glioma tissues compared with that in normal tissues. The SOX2 expression is higher in high grade (stage III and stage IV) glioma tissues than that in low grade (stage I and stage II) glioma tissues. GAPDH is the internal control. ^** P < 0.01 and ^*** P < 0.001. b The relative expression of SOX2 decreases in U251 and U 87 cells in si-SOX2 group. GAPDH is the internal control. ^** P < 0.01 compared with si-NC group. c-d The cell viability of U251 and U87 cells decreases in si-SOX2 group. OD: optical density. ^* P < 0.05 compared with si-NC group. e The invasion and migration of U251 and U87 cells decrease in si-SOX2 group. Scale bar: 100 μm. f The histogram of invasion and migration assay. ^* P < 0.05, ^** P < 0.01 compared with si-NC group. Each experiment was performed in triplicate and measured for 3 times