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Fig. 5 | Molecular Cancer

Fig. 5

From: LncRNA PTAR promotes EMT and invasion-metastasis in serous ovarian cancer by competitively binding miR-101-3p to regulate ZEB1 expression

Fig. 5

PTAR negatively regulates miR-101 expression and activity in OvCa cells. a, b The expression of PTAR and miR-101 in SKOV3 and A2780 cells after treatment with 10 ng/ml TGF-β1 for 48 h. c, d Analysis with qRT-PCR was used to assess the expression of PTAR and miR-101 in SKOV3 and A2780 cells in response to PTAR overexpression. e The knockdown efficiency of the sh-PTAR plasmid was confirmed by qRT-PCR analysis. f Expression of miR-101 was detected in OvCa cells in response to PTAR suppression. g The luciferase reporter activity of chimeric vectors carrying the luciferase gene and a fragment of PTAR containing the (WT) binding site or a mutated binding site for miR-101. h, i The luciferase activity of miR-101 sensor in OvCa cells transfected as specified. n = 5. *P < 0.05

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Molecular Cancer

ISSN: 1476-4598