Fig. 2

Clinical implication of miR-548k. a, Kaplan-Meier survival analysis of pooled ESCC cohort stratified by miR-548k amplification (n = 314; p = 0.038, log-rank test). b, The association of miR-548k RNA expression versus DNA copy number in TCGA ESCC cohort (p < 0.001, r = 0.51, Spearman’s Rank Correlation Coefficient analysis). c, Relative expression levels of miR-548k in 23 pairs of human ESCC tissues and matched adjacent normal tissues. d, Left, representative miRNA ISH photos of miR-548k expression in ESCC tissues and matched adjacent normal tissues. Scale bar: 100 μm. Right, MiRNA in situ hybridization (ISH) score in ESCC tissues and matched adjacent normal tissues (n = 185). e, Kaplan-Meier survival analysis of all ESCC patients stratified by miR-548k expression level (n = 178; p = 0.002, log-rank test). f, Multivariate analysis of the hazard ratios (HR) showed that the upregulation of miR-548k may be an independent prognostic factor for the overall survival rate (by the Cox multivariate proportional hazard regression model). The HR is presented as the means (95% confidence interval, 95% CI)