Fig. 3

SPAG5 facilitates cell migration in HCC. a Cells with SPAG5 overexpression or silence were subjected to Transwell assays. The migrated cells were stained with 0.05% crystal violet and counted. The representative images and the fold change of cell migration were presented. b Invasion assays were performed to determine the effect of SPAG5 on cell invasion. c Proteins extracted from stable cell lines were subjected to western blot to detect the expressions of EMT-related markers, such as N-cadherin and MMP2. d Stable cells were injected into the mice via the tail vein. At day 40, the lungs were dissected. HE staining was performed to indicate the tumor metastasis in the lungs. Representative images and the statistical analyses were shown. All *P < 0.05, **P < 0.01