Fig. 5

SPAG5 interacts with CEP55. a A positive correlation between SPAG5 mRNA and CEP55 mRNA was found in TCGA cohort. b The correlation of SPAG5 mRNA and CEP55 mRNA in fresh HCC tissues was identified. c Patients with high expression of SPAG5 protein in SYSUCC cohort were frequently accompanied with high expression of CEP55 protein. d Cells were transfected with SPAG5 overexpression vector. The mRNA level of CEP55 was determined. e The protein expression of CEP55 was examined in cells with SPAG5 overexpression or knockdown. f Proteins extracted from HCC cells were incubated with antibody for CEP55 or SPAG5. After deposited by Protein A/G-agarose, samples were subjected to western blot to investigate the interaction. g Cells were fixed by 4% PFA and incubated with antibodies overnight at 4 °C. After stained by fluorescence secondary antibodies and DAPI, cells were observed under confocal fluorescence microscope. h Cells expressing SPAG5 were transfected with CEP55 siRNA for 24 h. The cell proliferation was examined by foci formation assays. i The impact of CEP55 in SPAG5-mediated cell migration was determined by Transwell assays. *P < 0.05